MARYVILLE, Tenn. (AP) — A car on a CSX train carrying a flammable and toxic substance derailed and caught fire in eastern Tennessee, prompting the evacuation of thousands of people within a 2-mile radius.

The fire was still burning around noon Thursday, Maryville City Manager Greg McClain said at a news conference.

Ten first responders received hospital treatment after breathing fumes, but otherwise officials said there were no reported injuries.

Blount County Fire Department Lt. Johnny Leatherwood said a call about the train derailment came in Wednesday night at 11:50 p.m. EDT in Maryville.

About 5,000 people in the area were being evacuated, along with several businesses. A manufacturing plant, Denso Manufacturing, closed down Thursday morning because of its proximity to the derailment, Blount County firefighter Kermit Easterling said.

Craig Camuso, CSX regional vice president for state government affairs, said the derailed car was carrying acrylonitrile. The company said it’s a hazardous material used in multiple industrial processes including making plastics, it’s flammable and it’s dangerous if inhaled.

According to the Environmental Protection Agency, acrylonitrile is a colorless liquid. The EPA says some effects of breathing acrylonitrile include headaches, dizziness, irritability and rapid heartbeat.

Blount County Memorial Hospital spokesman Josh West said the ten first responders were given oxygen, which is standard procedure after exposure to the chemical. He said they were not injured but were being held for observation.

He also said no local residents underwent treatment due to fumes.

Blount County Mayor Ed Mitchell asked residents near the derailment site not to drink well water for now. He said CSX will provide bottled water to residents at a local middle school.

Maryville City Manager Greg McClain added that there’s no indication yet whether well water has been affected by the incident.

He also advised evacuees to make plans to be away from home at least for Thursday night.

Camuso said the company is placing evacuees in hotels, will provide reimbursement when it sets up its outreach center and will provide gift cards for food and essentials to those who need them.

“We will continue to do that for as long as it takes,” he said.

The train was traveling from Cincinnati to Waycross, Georgia.

Camuso said it had 57 cars and two locomotives, and that 27 cars carried hazardous chemicals: nine with acrylonitrile, 16 with propane and two with asphalt. He said the cause of the derailment is not yet known.

A statement from the Federal Railroad Administration said the agency had investigators and hazmat inspectors at the scene.

“Once it is safe, FRA will begin a thorough investigation to determine the cause of the derailment,” the statement said.

The National Transportation Safety Board is not investigating the accident, but will monitor it and could send an investigator later, NTSB spokesman Terry Williams said in an email. He said the agency considers factors such as loss of life, injuries, environmental damage and new safety issues when deciding whether to launch an investigation.

On its Facebook page, the Blount County Sheriff’s Office said early Thursday that the evacuations could last from 24 to 48 hours.

A shelter for residents was set up at a local high school. Several residents there said they were not aware of the derailment until they got a call or someone knocked on their door early in the morning.

“We saw police going back and forth and emergency vehicles going back and forth on our road, but we didn’t know why until about 3 to 3:30,” Maryville resident John Trull said. “That’s when they told us. We didn’t hear anything (beforehand). We just saw some emergency vehicles go by and kind of wondered what was going on, and that’s about it.”

Brittany Parrott said she was awakened by a knock on her apartment door at about 4:30 a.m. Although she didn’t hear the derailment, she said she noticed the effects of it as she went outside.

“You could smell it in the air,” Parrott said. “I had a headache, I was feeling nauseated and lightheaded, all the symptoms.”

Maryville is a town of nearly 30,000 people located about 20 miles south of Knoxville and just outside the Great Smoky Mountains National Park.

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Associated Press writers Adrian Sainz in Memphis, Tennessee, and Rebecca Yonker in Louisville, Kentucky, contributed to this report.